Investigations funded by the Longer Life Foundation have resulted to date in 98 publications in peer-reviewed medical journals. Find them here.
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2015 Publications

  • Human urinary composition controls antibacterial activity of siderocalin

    Shields-Cutler RR, Crowley JR, Hung CS, Stapleton AE, Aldrich CC, Marschall J, Henderson JP
    The Journal of Biological Chemistry April 10, 2015

  • Promoting health and longevity through diet: from model organisms to humans

    Fontana L, Partridge L
    Cell, 2015 March 25; 161(1); 106-18

  • Calorie restriction, endothelial function and blood pressure homeostasis

    Spelta F, Bertozzi B, Cominacini L, Fontana L
    Vascul Pharmacol. 2015 Feb-Mar, 65-6:1-2.

  • Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population

    Levine M, Suarez J, Brandhorst S, Balasubramanian, Cheng CW, Madia F, Fontana L, Mirisola MP, Wan J, Passarino G, Kennedy BK, Wei M, Cohen P, Crimmins E, Longo VD
    Cell Metabolism 2014 Mar 4;19(3):407–17

2014 Publications

  • Energy requirements in nonobese men and women: results from CALERIE

    Redman LM, Kraus WE, Bhapkar M, Das SK, Racette SB, Martin CK, Fontana L, Wong WW, Robert SB, Ravussin E, for the CALERIE Study Group
    Am J Clin Nutr Jan 2014

  • Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population

    Levine ME, Suarez JA, Brandhorst S, Balasubramanian P; Cheng CW, Madia F, Fontana L, Mirisola MG, Guevara-Aguirre J, Wan J, Passarino G, Kennedy BK, Wei M, Cohen P, Crimmins EM, Longo, VD
    Cell Metabolism 19, 407-17, March 4, 2014   

  • Optimal body weight for health and longevity: bridging basic, clinical, and population research

    Fontana L, Hu FB
    Aging Cell (2014) pp 1-10

  • Postprandial plasma incretin hormones in exercise-trained versus untrained subjects

    Weiss EP, Royer NK, Fisher JS, Holloszy JO, Fontana L
    Med Sci Sports Exerc 2014 Jun; 46(6): 1098-103

  • Cytochrome P450 gene variants, race and mortality among clopidogrel-treated patients after acute myocardial infarction

    Cresci S, Depta JP, Lenzini PA, Li AY, Lanfear DE, Province MA, Spertus JA,. Bach RG
    Circ Cardiovasc Genet 2014 Jun;7(3):277-86

  • Medical research: treat ageing

    Fontana L, Kennedy BK, Longo VD, Seals D, Melov S
    Nature 2014 Jul 24;511(7510):405-7

  • CHRNA5 variant predicts smoking cessation in patients with acute myocardial infarction

    Chen LS, Bach RG, Lenzini PA, Spertus JA, Bierut LJ, Cresci S
    Nicotine Tob Res 2014 Sep;16(9):1224-31

  • Urinary concentrations of aquaporin-1 and perilipin-2 in patients with renal cell carcinoma correlate with tumor size and stage but not grade

    Morrissey JJ, Mobley J, Song J, Vetter J, Luo J, Bhayani S, Figenshau RS , Kharasch ED
    Urology 2014 Jan;83(1): 256

  • What are the roles of calorie restriction and diet quality in promoting healthy longevity?

    Rizza W, Veronese N, Fontana L
    Ageing Research Reviews 2014 Jan; 13:38-45   

2013 Publications

  • Amyloid imaging and CSF biomarkers in predicting cognitive impairment up to 7.5 years later

    Roe CM, Fagan AM, Grant EA, Hassenstab J, Moulder KL, Maue Dreyfus D; Sutphen CL, Benzinger TL, Mintun MA, Holtzman DM, Morris JC
    Neurology 2013 May 7;80(19):1784-91

  • Assessing the diagnostic accuracy (DA) of the Spanish version of the informant-based AD8 questionnaire

    Carnero Pardo C, de la Vega Cotarelo R, López Alcalde S, Martos Aparicio C, Vilchez Carrillo R, Mora Gavilán E, Galvin JE
    Neurologia 2013 Mar;28(2):88-94

  • “Deficiency” of mitochondria in muscle does not cause insulin resistance

    Holloszy JO
    Diabetes 2013 Apr;62 (4): 1036-1040

  • Calorie restriction in humans inhibits the PI3K/AKT pathway and induces a younger transcription profile

    Mercken EM, Crosby SD, Lamming DW, JeBailey L, Krzysik-Walker S, Villareal DT, Capri M, Franceschi C, Zhang Y, Becker K, Sabatini DM, de Cabo R, Fontana L
    Aging Cell 2013 Aug;12(4):645-51

  • Protein restriction cycles reduce IGF-1 and phosphorylated Tau, and improve behavioral performance in an Alzheimer's disease mouse model

    Parrella E, Maxim T, Maialetti F, Zhang L, Wan J, Wei M, Cohen P, Fontana L, Longo VD
    Aging Cell 2013 Apr;12(2):257-6

  • Identification of a metabolic signature for multidimensional impairment and mortality risk in hospitalized older patients

    Fontana L, Addante F, Copetti M, Paroni G, Fontana A, Sancarlo D, Pellegrini F, Ferrucci L, Pilotto A
    Aging Cell 2013 Jun;12(3):459-66

  • Will calorie restriction work in humans?

    Cava E, Fontana L
    Aging 2013 Jul;5(7):507-14

  • Serum from humans on long-term calorie restriction enhances stress resistance in cell culture

    Omodei D, Licastro D, Salvatore F, Crosby SD, Fontana L
    Aging 2013 Aug;5(8):599-606

  • Energy efficiency as a unifying principle for human, environmental, and global health

    Fontana L, Atella V, Kammen DM
    F1000Research 2013, 2:101

  • Multiple dietary supplements do not affect metabolic and cardiovascular health

    Soare A, Weiss EP, Holloszy JO, Fontana L
    Aging 2013 Sep 4 (Epub)

  • Dietary protein restriction inhibits tumor growth in human xenograft models

    Fontana L, Adelaiye RM, Rastelli AL, Miles KM, Ciamporcero E, Longo VD, Nguyen H, Vessella R, Pili R
    Oncotarget 2013 Dec;4(12):2451-61 

  • Association between specific adipose tissue CD4+ T-cell populations and insulin resistance in obese individuals

    Fabbrini E, Cella M, McCartney SA, Fuchs A, Abumrad NA, Pietka TA, Chen Z, Finck BN, Han DH, Magkos F, Conte C, Bradley D, Fraterrigo G, Eagon JC, Patterson BW, Colonna M, Klein S
    Gastroenterology 2013 Aug;145(2):366-74

  • CSF biomarkers of Alzheimer disease: “noncognitive” outcomes

    Roe CM, Fagan AM, Grant EA, Holtzman DM, Morris JC
    Neurology 2013 Dec 3;81(23):2028-31

  • Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography

    Yoshino J, Imai S
    Methods Mol Biol 2013;1077:203-15

2012 Publications

  • An exploration of salivation patterns in normal weight and obese children

    Aspen VA, Stein RI, Wilfley DE
    Appetite 2012 Apr 58(2):539-42

  • Effect of weight loss on the rate of muscle protein synthesis during fasted and fed conditions in obese older adults

    Villareal DT, Smith GI, Shah K, Mittendorfer B
    Obesity 2012 Sep;20 (9):1780-6

  • Adrenergic-pathway gene variants influence beta-blocker-related outcomes after acute coronary syndrome in a race-specific manner

    Cresci S, Dorn GW 2nd, Jones PG, Beitelshees, AL, Li AY, Lenzini PA, Province MA, Spertus JA, Lanfear DE
    Journal of the American College of Cardiology 2012 Sep 4;60(10):898-907

  • Growth factors, nutrient signaling, and cardiovascular aging

    Fontana L, Vinciguerra M, Longo VD
    Circulation Research 2012 Apr 13;110(8):1139-50

  • Preferential reductions in intermuscular and visceral adipose tissue with exercise-induced weight loss as compared to calorie restriction

    Murphy JC, McDaniel JL, Mora K, Villareal DT, Fontana L, Weiss EP
    Journal of Applied Physiology 2012 Jan;112(1):79-85

  • Klotho locus, metabolic traits, and serum hemoglobin in hospitalized older patients: a genetic association analysis

    Paroni G, Seripa D, Panza F, Addante F, Copetti M, D'Onofrio G, Pellegrini F, Fontana L, Pilotto A
    Age (Dordr) 2012 Aug;34(4):949-68

  • Sexually dimorphic effect of aging on skeletal muscle protein synthesis

    Smith GI, Reeds DN, Hall AM, Chambers KT, Finck BN, Mittendorfer B
    Biology of Sex Differences 2012 May 23;3(1):11

  • Caloric restriction may reverse age-related autonomic decline in humans

    Stein PK, Soare A, Meyer TE, Cangemi R, Holloszy JO, Fontana L
    Aging Cell 2012 Aug;11(4):644-50

  • Dehydroepiandrosterone replacement therapy in older adults improves indices of arterial stiffness

    Weiss EP, Villareal DT, Ehsani AA, Fontana L, Holloszy JO
    Aging Cell 2012 Oct;11(5):876-84

  • Resveratrol supplementation does not improve metabolic function in nonobese women with normal glucose tolerance

    Yoshino J, Conte C, Fontana L, Mittendorfer B, Imai S, Schechtman KB, Gu CC, Kunz I, Rossi Fanelli F, Patterson BW, Klein S
    Cell Metabolism 2012 Nov 7;16(5):658-64

  • [The reliability and validity of Chinese version of AD8]. (article in Chinese)

    Li T, Wang HL, Yang YH, Galvin JE, Morris JC, Yu X
    Zhonghua Nei Ke Za Zhi 2012 Oct;51(10):777-80

  • Predicting road test performance in adults with cognitive or visual impairment referred to a Veterans Affairs Medical Center driving clinic

    Niewoehner PM, Henderson RR, Dalchow J, Beardsley TL, Stern RA, Carr DB
    Journal of the American Geriatric Society 2012 Nov;60(11):2070-4

2011 Publications

  • Ginseng and ginsenoside re do not improve ß-cell function or insulin sensitivity in overweight and obese subjects with impaired glucose tolerance or diabetes

    Reeds DN, Holloszy JO, Patterson BW, Polonsky KS, Okunade A, Klein S
    Diabetes Care Volume 34, May 2011 1071-6

  • Intermittent supplementation with rapamycin as a dietary restriction mimetic

    Longo VD, Fontana L
    Aging (Albany NY) 2011 Nov;3(11):1039-40

  • Application of AD8 questionnaire to screen very mild dementia in Taiwanese

    Yang YH, Galvin JE, Morris JC, Lai CL, Chou MC, Liu CK
    American Journal of Alzheimer’s Disease & Other Dementias 2011 Mar;26(2):134-8

  • Caloric restriction: powerful protection for the aging heart and vasculature

    Weiss EP, Fontana L
    American Journal of Physiology – Heart and Circulatory Physiology 2011 Oct;301(4):H1205-19

  • Regular multicomponent exercise increases physical fitness and muscle protein anabolism in frail, obese, older adults

    Villareal DT, Smith GI, Sinacore DR, Shah K, Mittendorfer B
    Obesity 2011 Feb;19(2):312-8

  • Dietary omega-3 fatty acid supplementation increases the rate of muscle protein synthesis in older adults: a randomized controlled trial

    Smith GI, Atherton P, Reeds DN, Mohammed BS, Rankin D, Rennie MJ, Mittendorfer B
    American Journal of Clinical Nutrition 2011 Feb;93(2):402-12

  • Omega-3 polyunsaturated fatty acids augment the muscle protein anabolic response to hyperinsulinaemia-hyperaminoacidaemia in healthy young and middle-aged men and women

    Smith GI, Atherton P, Reeds DN, Mohammed BS, Rankin D, Rennie MJ, Mittendorfer B
    Clinical Science (London, England) 2011 Sep;121(6):267-78

  • Calorie restriction and prevention of age-associated chronic disease

    Omodei D, Fontana L
    FEBS Letters 2011 Jun 6;585(11):1537-42

  • Reduced bone mineral density is not associated with significantly reduced bone quality in men and women practicing long-term calorie restriction with adequate nutrition

    Villareal DT, Kotyk J,J Armamento-Villareal RC, Kenguva V, Seaman P, Shahar A, Wald MJ, Kleerekoper M, Fontana L
    Aging Cell 2011 Feb; 10(1):96-102

  • Long-term calorie restriction, but not endurance exercise, lowers core body temperature in humans

    Soare A, Cangemi R, Omodei D, Holloszy JO, Fontana L
    Aging 2011 Apr;3(4):374-9

  • Peroxisome proliferator-activated receptor pathway gene polymorphism associated with extent of coronary artery disease in patients with type 2 diabetes in the bypass angioplasty revascularization investigation 2 diabetes trial

    Cresci S, Wu J, Province MA, Spertus JA, Steffes M, McGill JB, Alderman EL, Brooks MM, Kelsey SF, Frye RL, Bach RG; BARI 2D Study Group
    Circulation 2011 Sep 27;124(13):1426-34

  • Dementia screening, biomarkers and protein misfolding: Implications for public health and diagnosis

    Galvin JE
    Prion 2011 Jan-Mar;5(1):16-21

  • AD8-Brazil: cross-cultural validation of the ascertaining dementia interview in Portuguese

    Correia CC, Lima F, Junqueira F, Campos MS, Bastos O, Petribú K, Laks J, Galvin JE
    Journal of Alzheimer’s Disease.2011;27(1):177-85

  • Dissecting systemic control of metabolism and aging in the NAD World: the importance of SIRT1 and NAMPT-mediated NAD biosysthesis

    Imai S
    FEBS Lett 2011 Jun 6;585(11): 1657-62

  • Predicting road test performance in drivers with dementia

    Carr DB, Barco PP, Wallendorf MJ, Snellgrove CA, Ott BR
    Journal of the American Geriatric Society 2011 Nov;59(11):2112-7

  • Nicotinamide mononucleotide, a key NAD+ intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice.

    Yoshino J, Mills KF, Yoon JY and Imai S.
    Cell Metab. 2011 Oct 5;14(4):528-36.

2010 Publications

  • Ten years of NAD-dependent SIR2 family deacetylases: implications for metabolic diseases

    Imai S, Guarente L
    Trends Pharmacol Sci 2010 May;31(5):212-20

  • Relationship of dementia screening tests with biomarkers of Alzheimer's disease

    Galvin JE, Fagan AM, Holtzman DM, Mintun MA, Morris JC
    Brain. 2010 Nov;133(11):3290-300

  • A microRNA expression signature for cervical cancer prognosis

    Hu X, Schwarz JK, Lewis JS Jr, Huettner PC, Rader JS, Deasy JO, Grigsby PW, Wang X
    Cancer Research 2010 Feb 15;70(4):1441-8

  • Extending healthy life span – from yeast to humans

    Fontana L, Partridge L, Longo VD
    Science 2010 Apr 16;328(5976):321-6

  • Long-term effects of calorie restriction on serum sex-hormones concentrations in men

    Cangemi R, Friedmann, AJ, Holloszy JO, Fontana L
    Aging Cell 2010 Apr;9(2):236-42

  • Calorie restriction and cancer prevention: metabolic and molecular mechanisms

    Longo VD, Fontana L
    Trends in Pharmacological Sciences 2010 Feb;31(2):89-98

  • Effects of long-term calorie restriction and endurance exercise on glucose tolerance, insulin action, and adipokine production

    Fontana L, Klein S, Holloszy JO
    Age 2010 Mar;32(1):97–108

  • TRB3 function in cardiac endoplasmic reticulum stress

    Avery J, Etzion S, DeBosch BJ, Jin X, Lupu TS, Beitinjaneh B, Grand J, Kovacs A, Sambandam N, Muslin AJ
    Circulation Research 2010 May 14;106(9):1516-23

  • A possibility of nutriceuticals as an anti-aging intervention: activation of sirtuins by promoting mammalian NAD biosynthesis

    Imai, S
    Pharmacological Research 2010 Jul;62(1):42-7

  • Organizational support and volunteering benefits for older adults

    Tang F, Choi E, Morrow-Howell N
    The Gerontologist 2010 Oct;50(5):603-12

2009 Publications

  • Neuroendocrine factors in the regulation of inflammation: excessive adiposity and calorie restriction

    Fontana L
    Experimental Gerontology 2009 Jan-Feb;44(1-2):41-5

  • “Clocks” in the NAD world: NAD as a metabolic oscillator for the regulation of metabolism and aging

    Imai, S
    Biochimica et Biophysica Acta 2010 Aug;1804(8):1584-90

  • Driving retirement in older adults with dementia

    Croston J, Meuser TM, Berg-Weger M, Grant EA, Carr DB
    Topics in Geriatric Rehabilitation 2009 Apr 1;25(2): 154-62

  • The scientific basis of caloric restriction leading to longer life

    Fontana L
    Current Opinion in Gastroenterology 2009 Mar;25(2):144-50

  • Modulating human aging and age-associated diseases

    Fontana L
    Biochimca et Biophysica Acta 2009 Oct;1790 (10):1133-8

  • SIRT1 and caloric restriction: an insight into possible trade-offs between robustness and frailty

    Imai S
    Current Opinion in Clinical Nutrition and Metabolic Care 2009 Jul;12(4):350-6

  • Who benefits from volunteering: Variations in perceived benefits

    Morrow-Howell N, Hong SI, Tang F
    The Gerontologist 2009 Feb;49 (1):91-102

  • Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis

    Ramsey KM, Yoshino J, Brace CS, Abrassart D, Kobayashi Y, Marcheva B, Hong HK, Chong JL, Buhr ED, Lee C,Takahashi JS, Imai S, Bass J
    Science 2009 May 1;324(5927): 651-4

2008 Publications

  • MAPK signalling in cardiovascular health and disease: molecular mechanisms and therapeutic targets

    Muslin AJ
    Clinical Science (London, England) 2008 Oct;115(7):203-18

  • The anticonvulsant ethosuximide disrupts sensory function to extend C. elegans lifespan

    Collins JJ, Evason K, Pickett CL, Schneider DL, Kornfeld K
    PLoS Genetics 2008 Oct;4(10): e1000230

  • The measurement and analysis of age-related changes in Caenorhabditis elegans

    Collins JJ, Huang C, Hughes S, Kornfeld K
    WormBook 2008 Jan 24:1-21

  • Reduction of overweight and eating disorder symptoms via the Internet in adolescents: A randomized controlled trial

    Doyle AC, Goldschmidt A, Huang C, Winzelberg AJ, Taylor CB, Wilfley DE
    Journal of Adolescent Health 2008 Aug;43 (2): 172-9

  • Valproic acid extends Caenorhabditis elegans lifespan

    Evason K, Collins JJ, Huang C, Hughes S, Kornfeld K
    Aging Cell 2008 Jun;7(3):305-17

  • Long-term effects of calorie or protein restriction on serum IGF-1 and IGFBP-3 concentration in humans

    Fontana L, Weiss EP, Villareal DT, Klein S, Holloszy JO
    Aging Cell 2008 Oct;7(5):681-7

  • Translational approaches to addressing complex genetic pathways in colorectal cancer

    Early DS, Fontana L, Davidson NO
    Translational Research 2008 Jan;151(1):10-6

  • Calorie restriction and cardiometabolic health

    Fontana L
    European Journal of Cardiovascular Prevention and Rehabilitation 2008 Feb;15(1):3-9

  • Changes in weight and health behaviors from freshman through senior year of college

    Racette, SB, Deusinger SS, Strube MJ, Highstein GR, Deusinger RH
    J Nutr Educ Behav 2008 Jan-Feb;40(1):39-42

2007 Publications

  • Long-term low-calorie low-protein vegan diet and endurance exercise are associated with low cardiometabolic risk

    Fontana L, Meyer TE, Klein S. Holloszy JO
    Rejuvenation Res 2007 Jun;10(2):225-34

  • Aging, adiposity and calorie restriction

    Fontana L, Klein S
    JAMA 2007 Mar 7;297(9):986-94

  • Evaluation of cognitive impairment in older adults: combining brief informant and performance measures

    Galvin JE, Roe CM, Morris JC
    Archives of Neurology 2007 May,64(5):718-24

  • Patient’s rating of cognitive ability: using the AD8, a brief informant interview, as a self-rating tool to detect dementia

    Galvin JE, Roe CM, Coats MA, Morris JC
    Archives of Neurology 2007 May;64(5):725-30

  • Quantifying the usefulness of CT in evaluating seniors with abdominal pain

    Lewis LM, Klippel AP, Bavolek RA, Ross LM, Scherer TM, Banet GA
    European Journal of Radiology 2007 Feb;61(2):290-6

  • Productive engagement and late life physical and mental health: Findings from a nationally representative panel study

    Hinterlong JE, Morrow-Howell N, Rozario PA
    Research on Aging 2007 Jul;29(4):348-70

2006 Publications

  • Effects of anticonvulsant drugs on life span

    Kornfeld K, Evason K
    Archives of Neurology 2006 Apr;63(4):491-6

  • Pharmacology of delayed aging and extended lifespan of Caenorhabditis elegans

    Collins JJ, Evason K, Kornfeld K
    Experimental Gerontology 2006 Oct;41(10):1032-9

  • Validity and reliability of the AD8 informant interview in dementia

    Galvin JE, Roe CM, Xiong C, Morris JC
    Neurology 2006 Dec 12;67(11):1942-8

  • Effect of lifestyle intervention on metabolic coronary heart disease risk factors in obese older adults

    Villareal DT, Miller BV III, Banks M, Fontana L, Sinacore DR, Klein S
    The American Journal of Clinical Nutrition 2006 Dec;84(6):1317–23

2005 Publications

  • Anticonvulsant medications extend worm life-span

    Evason K, Huang C, Yamben I, Covey DF, Kornfeld K
    Science 2005 Jan 14;307(5707):258-62

  • The AD8: a brief informant interview to detect dementia

    Galvin JE, Roe CM, Powlishta KK, Coats MA, Muich SJ, Grant E, Miller JP, Storandt M, Morris JC
    Neurology 2005 Aug 23;65(4):559-64

  • Obesity in older adults: Technical review and position statement of the American Society for Nutrition and NAASO, The Obesity Society

    Villareal DT, Apovian CM, Kushner RF, Klein S
    Obesity Research 2005 Nov;13(11):1849-63

2003 Publications

  • Effects of volunteering on the well-being of older adults

    Morrow-Howell N, Hinterlong J, Rozario PA, Tang F
    The Journal of Gerontology Series B, Psychological Sciences and Social Sciences. 2003 May;58(3):S137-45

  • Predictors of mortality in younger and older patients with heart failure and preserved or reduced left ventricular ejection fraction

    Kerzner R, Gage BF, Freedland KE, Rich MW
    American Heart Journal 2003 Aug;146(2):286-90

  • Identifying factors associated with disability-related differences in breast cancer screening (United States)

    Schootman M, Jeffe DB
    Cancer Causes and Control 2003 Mar;14(2):97-107